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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DGKA Polyclonal antibody

DGKA Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11547

Print datasheet

Synonyms

EC:2.7.1.107, DGK-alpha, DGK alpha, DAGK1, DAGK

验证数据展示

  • IF Staining of Jurkat using CL488-11547

    IF Staining of Jurkat using CL488-11547

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 DGKA antibody (CL488-11547) at dilution of 1:200.

  • FC experiment of HeLa using CL488-11547

    FC experiment of HeLa using CL488-11547

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DGKA (CL488-11547) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11547-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11547 targets DGKA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen DGKA fusion protein Ag2075 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name diacylglycerol kinase, alpha 80kDa
Synonyms EC:2.7.1.107, DGK-alpha, DGK alpha, DAGK1, DAGK
Calculated Molecular Weight 735 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC023523
Gene Symbol DGKA
Gene ID (NCBI) 1606
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23743
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DGKA antibody CL488-11547Download protocol
FC protocol for CL Plus 488 DGKA antibody CL488-11547Download protocol
Standard Protocols
Click here to view our Standard Protocols