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验证数据展示

  • WB analysis of HepG2 using 60028-1-Ig

    WB analysis of HepG2 using 60028-1-Ig

    WB result of DHCR24 antibody (60028-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHCR24 transfected HepG2 cells.

  • WB analysis using 60028-1-Ig

    WB analysis using 60028-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60028-1-Ig (DHCR24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 60028-1-Ig

    IHC staining of human prostate cancer using 60028-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DHCR24 antibody included
  • IHC staining of human prostate cancer using 60028-1-Ig

    IHC staining of human prostate cancer using 60028-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DHCR24 antibody included

DHCR24 Monoclonal antibody

DHCR24 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60028-1-Ig

产品说明书

CloneNo. 2F6E11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

DHCR24, Diminuto/dwarf1 homolog, Seladin 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DHCR24 Ready-To-Use IHC Kit. DHCR24 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60028-1-Ig targets DHCR24 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 DHCR24 fusion protein Ag0756 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 24-dehydrocholesterol reductase
别名 DHCR24, Diminuto/dwarf1 homolog, Seladin 1
计算分子量 60 kDa, 516 aa
观测分子量 60 kDa
GenBank蛋白编号BC004375
基因名称 DHCR24
Gene ID (NCBI) 1718
RRIDAB_874115
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15392
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The dhcr24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).

实验方案

Product Specific Protocols
WB protocol for DHCR24 antibody 60028-1-IgDownload protocol
IHC protocol for DHCR24 antibody 60028-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols