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DHCR24 Monoclonal antibody, PBS Only (Detector)

DHCR24 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F6E11

Cat no : 60028-1-PBS

Print datasheet

Synonyms

DHC24, EC:1.3.1.72, Delta(24)-sterol reductase, 3-beta-hydroxysterol Delta-24-reductase, 2F6E11

验证数据展示

  • WB analysis of HepG2 using 60028-1-Ig (same clone as 60028-1-PBS)

    WB analysis of HepG2 using 60028-1-Ig (same clone as 60028-1-PBS)

    WB result of DHCR24 antibody (60028-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHCR24 transfected HepG2 cells. This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

  • WB analysis using 60028-1-Ig (same clone as 60028-1-PBS)

    WB analysis using 60028-1-Ig (same clone as 60028-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60028-1-Ig (DHCR24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

    IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DHCR24 antibody included
  • IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

    IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DHCR24 antibody included
  • Cytometric bead array standard curve of MP51020-1

    Cytometric bead array standard curve of MP51020-1

    Cytometric bead array standard curve of MP51020-1, DHCR24 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60028-2-PBS. Detection antibody: 60028-1-PBS. Standard:Ag0756. Range: 0.098-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy DHCR24 Ready-To-Use IHC Kit. DHCR24 primary antibody included.

产品信息

60028-1-PBS targets DHCR24 as part of a matched antibody pair:

MP51020-1: 60028-2-PBS capture and 60028-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen DHCR24 fusion protein Ag0756 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 24-dehydrocholesterol reductase
Synonyms DHC24, EC:1.3.1.72, Delta(24)-sterol reductase, 3-beta-hydroxysterol Delta-24-reductase, 2F6E11
Calculated Molecular Weight 60 kDa, 516 aa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC004375
Gene Symbol DHCR24
Gene ID (NCBI) 1718
RRIDAB_874115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15392
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The dhcr24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).