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验证数据展示

  • WB analysis of LNCaP using 67977-1-Ig

    WB analysis of LNCaP using 67977-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67977-1-Ig

    WB analysis of HSC-T6 using 67977-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DHODH Monoclonal antibody

DHODH Monoclonal Antibody for WB, ELISA
Cat No. 67977-1-Ig

Print datasheet

CloneNo. 2B2C6
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

DHOdehase, DHODH, dihydroorotate dehydrogenase, Dihydroorotate oxidase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67977-1-Ig targets DHODH in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivitymouse, monkey
Immunogen DHODH fusion protein Ag6649 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dihydroorotate dehydrogenase
Synonyms DHOdehase, DHODH, dihydroorotate dehydrogenase, Dihydroorotate oxidase
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC065245
Gene Symbol DHODH
Gene ID (NCBI) 1723
RRIDAB_2918726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02127
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

实验方案

Product Specific Protocols
WB protocol for DHODH antibody 67977-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
monkeyWB

Vet Res

Vidofludimus inhibits porcine reproductive and respiratory syndrome virus infection by targeting dihydroorotate dehydrogenase

Authors - Yuanqi Yang
View Article
mouseWB

ACS Nano

Engineering Biodegradable Hollow Silica/Iron Composite Nanozymes for Breast Tumor Treatment through Activation of the "Ferroptosis Storm"

Authors - Panpan Xue
View Article