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验证数据展示

  • WB analysis of LNCaP using 67977-1-Ig

    WB analysis of LNCaP using 67977-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67977-1-Ig

    WB analysis of HSC-T6 using 67977-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DHODH Monoclonal antibody

DHODH Monoclonal Antibody for WB, ELISA
Cat No. 67977-1-Ig

产品说明书

CloneNo. 2B2C6
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat and More (1)

应用

WB, ELISA

别名

DHOdehase, DHODH, dihydroorotate dehydrogenase, Dihydroorotate oxidase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67977-1-Ig targets DHODH in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat
文献引用反应性mouse, monkey
免疫原 DHODH fusion protein Ag6649 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dihydroorotate dehydrogenase
别名 DHOdehase, DHODH, dihydroorotate dehydrogenase, Dihydroorotate oxidase
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC065245
基因名称 DHODH
Gene ID (NCBI) 1723
RRIDAB_2918726
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ02127
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

实验方案

Product Specific Protocols
WB protocol for DHODH antibody 67977-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
monkeyWB

Vet Res

Vidofludimus inhibits porcine reproductive and respiratory syndrome virus infection by targeting dihydroorotate dehydrogenase

Authors - Yuanqi Yang
View Article
mouseWB

ACS Nano

Engineering Biodegradable Hollow Silica/Iron Composite Nanozymes for Breast Tumor Treatment through Activation of the "Ferroptosis Storm"

Authors - Panpan Xue
View Article