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验证数据展示

  • WB analysis of LNCaP using 67977-1-Ig (same clone as 67977-1-PBS)

    WB analysis of LNCaP using 67977-1-Ig (same clone as 67977-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67977-1-Ig (same clone as 67977-1-PBS)

    WB analysis of HSC-T6 using 67977-1-Ig (same clone as 67977-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50416-1

    Cytometric bead array standard curve of MP50416-1

    Cytometric bead array standard curve of MP50416-1, DHODH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67977-2-PBS. Detection antibody: 67977-1-PBS. Standard:Ag6649. Range: 1.563-100 ng/mL.

DHODH Monoclonal antibody, PBS Only (Detector)

DHODH Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 67977-1-PBS

产品说明书

CloneNo. 2B2C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67977-1-PBS targets DHODH as part of a matched antibody pair:

MP50416-1: 67977-2-PBS capture and 67977-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DHODH fusion protein Ag6649 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dihydroorotate dehydrogenase
别名 PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC065245
基因名称 DHODH
Gene ID (NCBI) 1723
RRIDAB_2918726
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ02127
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).