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验证数据展示

  • FC experiment of HeLa using CL488-67998

    FC experiment of HeLa using CL488-67998

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DHPS (CL488-67998, Clone:1E4F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated DHPS Monoclonal antibody

DHPS Monoclonal Antibody for FC (Intra)
Cat No. CL488-67998

产品说明书

CloneNo. 1E4F5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

MIG13, EC:2.5.1.46, DS, DHS, 1E4F5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67998-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67998 targets DHPS in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 DHPS fusion protein Ag31046 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deoxyhypusine synthase
别名 MIG13, EC:2.5.1.46, DS, DHS, 1E4F5
计算分子量 41 kDa
GenBank蛋白编号BC014016
基因名称 DHPS
Gene ID (NCBI) 1725
RRIDAB_2923853
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49366
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DHPS antibody CL488-67998Download protocol
Standard Protocols
Click here to view our Standard Protocols