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CoraLite® Plus 488-conjugated DHPS Monoclonal antibody

DHPS Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4F5

Cat no : CL488-67998

Print datasheet

Synonyms

MIG13, EC:2.5.1.46, DS, DHS, 1E4F5

验证数据展示

  • FC experiment of HeLa using CL488-67998

    FC experiment of HeLa using CL488-67998

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DHPS (CL488-67998, Clone:1E4F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67998-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67998 targets DHPS in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen DHPS fusion protein Ag31046 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name deoxyhypusine synthase
Synonyms MIG13, EC:2.5.1.46, DS, DHS, 1E4F5
Calculated Molecular Weight 41 kDa
GenBank Accession NumberBC014016
Gene Symbol DHPS
Gene ID (NCBI) 1725
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49366
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DHPS antibody CL488-67998Download protocol
Standard Protocols
Click here to view our Standard Protocols