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DHX32 Monoclonal antibody

DHX32 Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2A4G9

Cat no : 68306-1-Ig

Print datasheet

Synonyms

HuDDX32, EC:3.6.4.13, DHLP1, DEAD/H helicase-like protein 1, DDX32

验证数据展示

  • WB analysis using 68306-1-Ig

    WB analysis using 68306-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 68306-1-Ig

    WB analysis of A549 using 68306-1-Ig

    WB result of DHX32 antibody (68306-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX32 transfected A549 cells.

  • FC experiment of HeLa using 68306-1-Ig

    FC experiment of HeLa using 68306-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug DHX32 Monoclonal antibody (68306-1-Ig, Clone:2A4G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inA431 cells, U2OS cells, rat testis tissue, HeLa cells, A549 cells, mouse testis tissue, MCF-7 cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68306-1-Ig targets DHX32 in WB, FC (Intra), ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA, FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen DHX32 fusion protein Ag13956 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 32
Synonyms HuDDX32, EC:3.6.4.13, DHLP1, DEAD/H helicase-like protein 1, DDX32
Calculated Molecular Weight 743 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC002473
Gene Symbol DHX32
Gene ID (NCBI) 55760
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L7V1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHX32, an RNA helicase, belongs to the DEAH box RNA helicase family by virtue of its homology to the conserved helicase domain. DHX32 is abundantly expressed in human and murine models, participating in cell transcription and differentiation, and being capable of regulating T-cell apoptosis. DHX32 is also associated with some malignant tumors, such as lymphoma, colorectal cancer, and breast cancer (PMID: 35117530). DHX32 has 2 isoforms with molecular weights of 75 and 84 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for DHX32 antibody 68306-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols