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验证数据展示

  • WB analysis using 67853-1-Ig

    WB analysis using 67853-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67853-1-Ig (DHX37 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67853-1-Ig

    IF Staining of HeLa using 67853-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX37 antibody (67853-1-Ig, Clone: 2B12D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 67853-1-Ig

    IF Staining of HeLa using 67853-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX37 antibody (67853-1-Ig, Clone: 2B12D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

DHX37 Monoclonal antibody

DHX37 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67853-1-Ig

Print datasheet

CloneNo. 2B12D9

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67853-1-Ig targets DHX37 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen DHX37 fusion protein Ag29909 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 37
Synonyms DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517
Calculated Molecular Weight 1157 aa, 130 kDa
Observed Molecular Weight130-140 kDa
GenBank Accession NumberBC037964
Gene Symbol DHX37
Gene ID (NCBI) 57647
RRIDAB_2918611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IY37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHX37, also known as DDX37, KIAA1517. It is an ATP-bound RNA helicase that plays a role in the maturation of small ribosomal subunits in ribosomal biogenesis (PMID:30582406). The presence of DHX37 in early pre-ribosomal particles was monitored by a quality control pathway, and failure to recruit DHX37 resulted in pre-RRNA degradation. DHX37 binds directly to U3 small nucleolar RNA (snoRNA), and the catalytic activity of the helicase is required for dissociation of U3 small nucleolar RNA from the pre-ribosomal complex.In addition, it plays a role in early testicular development (PMID:31287541, PMID:31337883). It may also have an effect on brain development (PubMed:31256877). It is expressed in the fallopian tubes, ovaries, uterus, testes and brain (PMID: 31256877,PMID: 31337883).

实验方案

Product Specific Protocols
WB protocol for DHX37 antibody 67853-1-IgDownload protocol
IF protocol for DHX37 antibody 67853-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols