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验证数据展示

  • FC experiment of HeLa using CL594-67853

    FC experiment of HeLa using CL594-67853

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DHX37 (CL594-67853, Clone:2B12D9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated DHX37 Monoclonal antibody

DHX37 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67853

Print datasheet

CloneNo. 2B12D9

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Synonyms

DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67853-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67853 targets DHX37 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen DHX37 fusion protein Ag29909 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 37
Synonyms DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517
Calculated Molecular Weight 1157 aa, 130 kDa
Observed Molecular Weight130-140 kDa
GenBank Accession NumberBC037964
Gene Symbol DHX37
Gene ID (NCBI) 57647
RRIDAB_3084789
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IY37
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHX37, also known as DDX37, KIAA1517. It is an ATP-bound RNA helicase that plays a role in the maturation of small ribosomal subunits in ribosomal biogenesis (PMID:30582406). The presence of DHX37 in early pre-ribosomal particles was monitored by a quality control pathway, and failure to recruit DHX37 resulted in pre-RRNA degradation. DHX37 binds directly to U3 small nucleolar RNA (snoRNA), and the catalytic activity of the helicase is required for dissociation of U3 small nucleolar RNA from the pre-ribosomal complex.In addition, it plays a role in early testicular development (PMID:31287541, PMID:31337883). It may also have an effect on brain development (PubMed:31256877). It is expressed in the fallopian tubes, ovaries, uterus, testes and brain (PMID: 31256877,PMID: 31337883).

实验方案

Product Specific Protocols
FC protocol for CL594 DHX37 antibody CL594-67853Download protocol
Standard Protocols
Click here to view our Standard Protocols