验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, DU 145 cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, human cervical cancer tissue, rat testis tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14342-1-AP targets DIAPH3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DIAPH3 fusion protein Ag5656 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | diaphanous homolog 3 (Drosophila) |
| 别名 | MDia2, DRF3, Diaphanous-related formin-3, DIAP3 |
| 计算分子量 | 137 kDa |
| 观测分子量 | 125-137 kDa |
| GenBank蛋白编号 | BC048963 |
| 基因名称 | DIAPH3 |
| Gene ID (NCBI) | 81624 |
| RRID | AB_2092930 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NSV4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DIAPH3 antibody 14342-1-AP | Download protocol |
| IHC protocol for DIAPH3 antibody 14342-1-AP | Download protocol |
| IF protocol for DIAPH3 antibody 14342-1-AP | Download protocol |
| IP protocol for DIAPH3 antibody 14342-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Blood Membrane skeleton modulates erythroid proteome remodeling and organelle clearance.
| ||
Sci Adv Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice. | ||
Aging (Albany NY) The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer | ||
Cell Res Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.
| ||
EMBO J TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis. | ||
Elife Generation of stress fibers through myosin-driven reorganization of the actin cortex
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