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验证数据展示

  • IF Staining of HeLa using CL488-14342

    IF Staining of HeLa using CL488-14342

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DIAPH3 antibody (CL488-14342) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated DIAPH3 Polyclonal antibody

DIAPH3 Polyclonal Antibody for IF/ICC
Cat No. CL488-14342

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

diap3, DIAPH3, Diaphanous related formin 3, DKFZp434C0931, DKFZp686A13178, DRF3, FLJ34705, mDia2, Protein diaphanous homolog 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14342-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14342 targets DIAPH3 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 DIAPH3 fusion protein Ag5656 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diaphanous homolog 3 (Drosophila)
别名 diap3, DIAPH3, Diaphanous related formin 3, DKFZp434C0931, DKFZp686A13178, DRF3, FLJ34705, mDia2, Protein diaphanous homolog 3
计算分子量 137 kDa
观测分子量 125-137 kDa
GenBank蛋白编号BC048963
基因名称 DIAPH3
Gene ID (NCBI) 81624
RRIDAB_3083955
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSV4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DIAPH3 antibody CL488-14342Download protocol
Standard Protocols
Click here to view our Standard Protocols