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DICER1 Monoclonal antibody

DICER1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1C8E2

Cat no : 68375-1-Ig

Print datasheet

Synonyms

DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928

验证数据展示

  • WB analysis of U2OS using 68375-1-Ig

    WB analysis of U2OS using 68375-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68375-1-Ig (DICER1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 68375-1-Ig

    IP experiment of Jurkat using 68375-1-Ig

    IP result of anti-DICER1 (IP:68375-1-Ig, 4ug; Detection:68375-1-Ig 1:500) with Jurkat cells lysate 1560 ug.

  • IF Staining of HepG2 using 68375-1-Ig

    IF Staining of HepG2 using 68375-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DICER1 antibody (68375-1-Ig, Clone: 1C8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, Ramos cells, Sp2/0 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68375-1-Ig targets DICER1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen DICER1 fusion protein Ag30368 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dicer 1, ribonuclease type III
Synonyms DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928
Calculated Molecular Weight 219 kDa
Observed Molecular Weight240-250 kDa
GenBank Accession NumberNM_030621
Gene Symbol DICER1
Gene ID (NCBI) 23405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UPY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DICER1 functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. DICER1 cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, and producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation.

实验方案

Product Specific Protocols
WB protocol for DICER1 antibody 68375-1-IgDownload protocol
IF protocol for DICER1 antibody 68375-1-IgDownload protocol
IP protocol for DICER1 antibody 68375-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols