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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis of U2OS using 68375-1-Ig (same clone as 68375-1-PBS)

    WB analysis of U2OS using 68375-1-Ig (same clone as 68375-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68375-1-Ig (DICER1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68375-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 68375-1-Ig (same clone as 68375-1-PBS)

    IP experiment of Jurkat using 68375-1-Ig (same clone as 68375-1-PBS)

    IP result of anti-DICER1 (IP:68375-1-Ig, 4ug; Detection:68375-1-Ig 1:500) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68375-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68375-1-Ig (same clone as 68375-1-PBS)

    IF Staining of HepG2 using 68375-1-Ig (same clone as 68375-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DICER1 antibody (68375-1-Ig, Clone: 1C8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68375-1-PBS in a different storage buffer formulation.

DICER1 Monoclonal antibody, PBS Only

DICER1 Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 68375-1-PBS

产品说明书

CloneNo. 1C8E2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68375-1-PBS targets DICER1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原 DICER1 fusion protein Ag30368 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dicer 1, ribonuclease type III
别名 DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928
计算分子量 219 kDa
观测分子量240-250 kDa
GenBank蛋白编号NM_030621
基因名称 DICER1
Gene ID (NCBI) 23405
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UPY3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DICER1 functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. DICER1 cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, and producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation.