验证数据展示
经过测试的应用
Positive WB detected in | Raji cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10183-1-AP targets DIDO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | DIDO1 fusion protein Ag0237 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | death inducer-obliterator 1 |
别名 | death inducer obliterator 1, DATF-1, DATF1, DATF 1, C20orf158 |
计算分子量 | 59 kDa |
观测分子量 | 300-350 kDa |
GenBank蛋白编号 | BC000770 |
基因名称 | DIDO1 |
Gene ID (NCBI) | 11083 |
RRID | AB_2277375 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BTC0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DIDO1, also termed DATF-1, C20orf158, DATF1, KIAA0333 and DIO-1, is a putative transcription factor, weakly pro-apoptotic when overexpressed. It is a tumor suppressor. In mice, DIDO1 is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. The human DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis.
实验方案
Product Specific Protocols | |
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WB protocol for DIDO1 antibody 10183-1-AP | Download protocol |
IHC protocol for DIDO1 antibody 10183-1-AP | Download protocol |
IF protocol for DIDO1 antibody 10183-1-AP | Download protocol |
FC protocol for DIDO1 antibody 10183-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |