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DIDO1 Polyclonal antibody

DIDO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10183-1-AP

Print datasheet

Synonyms

death inducer obliterator 1, DATF-1, DATF1, DATF 1, C20orf158

验证数据展示

  • WB analysis of Raji using 10183-1-AP

    WB analysis of Raji using 10183-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10183-1-AP (DIDO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10183-1-AP

    IHC staining of human breast cancer using 10183-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DIDO1 antibody included
  • IHC staining of human breast cancer using 10183-1-AP

    IHC staining of human breast cancer using 10183-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DIDO1 antibody included
  • IF Staining of HepG2 using 10183-1-AP

    IF Staining of HepG2 using 10183-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DIDO1 antibody (10183-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DIDO1 Ready-To-Use IHC Kit. DIDO1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10183-1-AP targets DIDO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DIDO1 fusion protein Ag0237 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name death inducer-obliterator 1
Synonyms death inducer obliterator 1, DATF-1, DATF1, DATF 1, C20orf158
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 300-350 kDa
GenBank Accession NumberBC000770
Gene Symbol DIDO1
Gene ID (NCBI) 11083
RRIDAB_2277375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIDO1, also termed DATF-1, C20orf158, DATF1, KIAA0333 and DIO-1, is a putative transcription factor, weakly pro-apoptotic when overexpressed. It is a tumor suppressor. In mice, DIDO1 is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. The human DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis.

实验方案

Product Specific Protocols
WB protocol for DIDO1 antibody 10183-1-APDownload protocol
IHC protocol for DIDO1 antibody 10183-1-APDownload protocol
IF protocol for DIDO1 antibody 10183-1-APDownload protocol
FC protocol for DIDO1 antibody 10183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols