验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DIDO1 Ready-To-Use IHC Kit. DIDO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10183-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-10183 targets DIDO1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | DIDO1 fusion protein Ag0237 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | death inducer-obliterator 1 |
| 别名 | death inducer obliterator 1, DATF-1, DATF1, DATF 1, C20orf158 |
| 计算分子量 | 59 kDa |
| 观测分子量 | 300-350 kDa |
| GenBank蛋白编号 | BC000770 |
| 基因名称 | DIDO1 |
| Gene ID (NCBI) | 11083 |
| RRID | AB_3672431 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BTC0 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DIDO1, also termed DATF-1, C20orf158, DATF1, KIAA0333 and DIO-1, is a putative transcription factor, weakly pro-apoptotic when overexpressed. It is a tumor suppressor. In mice, DIDO1 is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. The human DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DIDO1 antibody CL488-10183 | Download protocol |
| FC protocol for CL Plus 488 DIDO1 antibody CL488-10183 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |