验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue |
Positive IHC detected in | human heart tissue, human kidney tissue, human testis tissue, human skin tissue, human lung tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
13816-1-AP targets DIXDC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse |
文献引用反应性 | human, rat |
免疫原 | DIXDC1 fusion protein Ag4904 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | DIX domain containing 1 |
别名 | CCD1, Coiled coil DIX1, Coiled coil protein DIX1, DIX domain containing 1, DIXDC1, Dixin, KIAA1735 |
计算分子量 | 683 aa, 77 kDa |
观测分子量 | 70-75 kDa |
GenBank蛋白编号 | BC033034 |
基因名称 | DIXDC1 |
Gene ID (NCBI) | 85458 |
RRID | AB_2091188 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q155Q3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for DIXDC1 antibody 13816-1-AP | Download protocol |
IHC protocol for DIXDC1 antibody 13816-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Neurobiol Wnt Signaling Pathways Are Dysregulated in Rat Female Cerebellum Following Early Methyl Donor Deficiency. | ||
Mol Carcinog The oncogenic role and regulatory mechanism of ACAA2 in human ovarian cancer |