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DIXDC1 Polyclonal antibody

DIXDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13816-1-AP

Print datasheet

Synonyms

CCD1, Coiled coil DIX1, Coiled coil protein DIX1, DIX domain containing 1, DIXDC1, Dixin, KIAA1735

验证数据展示

  • WB analysis of mouse skeletal muscle using 13816-1-AP

    WB analysis of mouse skeletal muscle using 13816-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13816-1-AP (DIXDC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 13816-1-AP

    IHC staining of human heart using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13816-1-AP (DIXDC1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 13816-1-AP

    IHC staining of human heart using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13816-1-AP (DIXDC1 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, human testis tissue, human skin tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13816-1-AP targets DIXDC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen DIXDC1 fusion protein Ag4904 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DIX domain containing 1
Synonyms CCD1, Coiled coil DIX1, Coiled coil protein DIX1, DIX domain containing 1, DIXDC1, Dixin, KIAA1735
Calculated Molecular Weight 683 aa, 77 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC033034
Gene Symbol DIXDC1
Gene ID (NCBI) 85458
RRIDAB_2091188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ155Q3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DIXDC1 antibody 13816-1-APDownload protocol
IHC protocol for DIXDC1 antibody 13816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Mol Neurobiol

Wnt Signaling Pathways Are Dysregulated in Rat Female Cerebellum Following Early Methyl Donor Deficiency.

Authors - Jérèmy Willekens
View Article
humanWB

Mol Carcinog

The oncogenic role and regulatory mechanism of ACAA2 in human ovarian cancer

Authors - Yahui Leng
View Article