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DLAT Recombinant antibody

DLAT Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240713C12

Cat no : 83654-3-RR

Print datasheet

Synonyms

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

验证数据展示

  • WB analysis using 83654-3-RR

    WB analysis using 83654-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83654-3-RR (DLAT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83654-3-RR

    IHC staining of human stomach cancer using 83654-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83654-3-RR (DLAT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with DLAT antibody included
  • IF Staining of HepG2 using 83654-3-RR

    IF Staining of HepG2 using 83654-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (83654-3-RR, Clone: 240713C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 83654-3-RR

    FC experiment of HepG2 using 83654-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DLAT Recombinant Antibody (83654-3-RR, Clone:240713C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83654-3-RR

    Affinity and Kinetic Characterization of 83654-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83654-3-RR against Human DLAT were performed. The affinity constant is 0.17 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, LNCaP cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DLAT Ready-To-Use IHC Kit. DLAT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83654-3-RR targets DLAT in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DLAT fusion protein Ag22831 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dihydrolipoamide S-acetyltransferase
Synonyms PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Calculated Molecular Weight 647 aa, 70 kDa
Observed Molecular Weight 69-70 kDa
GenBank Accession NumberBC039084
Gene Symbol DLAT
Gene ID (NCBI) 1737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10515
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DLAT antibody 83654-3-RRDownload protocol
IHC protocol for DLAT antibody 83654-3-RRDownload protocol
IF protocol for DLAT antibody 83654-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols