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DLC1 Monoclonal antibody, PBS Only

DLC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10H12

Cat no : 66894-1-PBS

Print datasheet

Synonyms

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12

验证数据展示

  • WB analysis using 66894-1-Ig (same clone as 66894-1-PBS)

    WB analysis using 66894-1-Ig (same clone as 66894-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66894-1-Ig (DLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • IF Staining of A375 using 66894-1-Ig (same clone as 66894-1-PBS)

    IF Staining of A375 using 66894-1-Ig (same clone as 66894-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

产品信息

66894-1-PBS targets DLC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen DLC1 fusion protein Ag24404 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name deleted in liver cancer 1
Synonyms ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12
Calculated Molecular Weight 171 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC054511
Gene Symbol DLC1
Gene ID (NCBI) 10395
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96QB1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Deleted in Liver Cancer 1 (DLC1) is a RhoGAP-containing tumor suppressor that inhibits angiogenesis by repressing VEGF production in epithelial cells. DLC1 is often down-regulated in a variety of cancers, such as those of the liver, lung, breast, stomach, brain, colon and prostate due to either genomic deletion or aberrant DNA methylation.