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验证数据展示

  • WB analysis using 67702-1-Ig (same clone as 67702-1-PBS)

    WB analysis using 67702-1-Ig (same clone as 67702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67702-1-Ig (same clone as 67702-1-PBS)

    WB analysis of HeLa using 67702-1-Ig (same clone as 67702-1-PBS)

    WB result of DLD antibody (67702-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DLD antibody included
  • IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DLD antibody included
  • Featured Product
  • KD/KO验证

DLD Monoclonal antibody, PBS Only

DLD Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67702-1-PBS

产品说明书

CloneNo. 2E7G5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DLD Ready-To-Use IHC Kit. DLD primary antibody included.

产品信息

67702-1-PBS targets DLD in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 DLD fusion protein Ag9680 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dihydrolipoamide dehydrogenase
别名 dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3
计算分子量 509 aa, 54 kDa
观测分子量54 kDa
GenBank蛋白编号BC018696
基因名称 DLD
Gene ID (NCBI) 1738
RRIDAB_2882894
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP09622
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.