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验证数据展示

  • WB analysis using 67702-1-Ig (same clone as 67702-1-PBS)

    WB analysis using 67702-1-Ig (same clone as 67702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67702-1-Ig (same clone as 67702-1-PBS)

    WB analysis of HeLa using 67702-1-Ig (same clone as 67702-1-PBS)

    WB result of DLD antibody (67702-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DLD antibody included
  • IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DLD antibody included
  • Featured Product
  • KD/KO Validated

DLD Monoclonal antibody, PBS Only

DLD Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67702-1-PBS

Print datasheet

CloneNo. 2E7G5

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DLD Ready-To-Use IHC Kit. DLD primary antibody included.

产品信息

67702-1-PBS targets DLD in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen DLD fusion protein Ag9680 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dihydrolipoamide dehydrogenase
Synonyms dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3
Calculated Molecular Weight 509 aa, 54 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberBC018696
Gene Symbol DLD
Gene ID (NCBI) 1738
RRIDAB_2882894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09622
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.