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  • Featured Product
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DLD Monoclonal antibody

DLD Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E7G5

Cat no : 67702-1-Ig

Print datasheet

Synonyms

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3

验证数据展示

  • WB analysis using 67702-1-Ig

    WB analysis using 67702-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67702-1-Ig

    WB analysis of HeLa using 67702-1-Ig

    WB result of DLD antibody (67702-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells.

  • IHC staining of human liver cancer using 67702-1-Ig

    IHC staining of human liver cancer using 67702-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLD antibody included
  • IHC staining of human liver cancer using 67702-1-Ig

    IHC staining of human liver cancer using 67702-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLD antibody included

经过测试的应用

Positive WB detected inHuH-7 cells, NIH/3T3 cells, HSC-T6 cells, HeLa cells, L02 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DLD Ready-To-Use IHC Kit. DLD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

67702-1-Ig targets DLD in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen DLD fusion protein Ag9680 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dihydrolipoamide dehydrogenase
Synonyms dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3
Calculated Molecular Weight 509 aa, 54 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberBC018696
Gene Symbol DLD
Gene ID (NCBI) 1738
RRIDAB_2882894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09622
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

实验方案

Product Specific Protocols
WB protocol for DLD antibody 67702-1-IgDownload protocol
IHC protocol for DLD antibody 67702-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

The signature of cuproptosis-related immune genes predicts the tumor microenvironment and prognosis of prostate adenocarcinoma

Authors - Kai Yao
View Article