验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy DLD Ready-To-Use IHC Kit. DLD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67702-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67702 targets DLD in IF-P applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | DLD fusion protein Ag9680 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | dihydrolipoamide dehydrogenase |
| Synonyms | dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3 |
| Calculated Molecular Weight | 509 aa, 54 kDa |
| GenBank Accession Number | BC018696 |
| Gene Symbol | DLD |
| Gene ID (NCBI) | 1738 |
| RRID | AB_2919524 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P09622 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DLD antibody CL488-67702 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |