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DLX3 Polyclonal antibody

DLX3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 13261-3-AP

Print datasheet

Synonyms

AI4, distal less homeobox 3, DLX3, Homeobox protein DLX 3, TDO

验证数据展示

  • WB analysis using 13261-3-AP

    WB analysis using 13261-3-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13261-3-AP (DLX3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 13261-3-AP

    IHC staining of human lung cancer using 13261-3-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13261-3-AP (DLX3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung cancer using 13261-3-AP

    IHC staining of human lung cancer using 13261-3-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13261-3-AP (DLX3 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inJAR cells, mouse lung tissue, NCCIT cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13261-3-AP targets DLX3 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen DLX3 fusion protein Ag3865 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name distal-less homeobox 3
Synonyms AI4, distal less homeobox 3, DLX3, Homeobox protein DLX 3, TDO
Calculated Molecular Weight 287 aa, 32 kDa
Observed Molecular Weight 32-38 kDa
GenBank Accession NumberBC028970
Gene Symbol DLX3
Gene ID (NCBI) 1747
RRIDAB_2292996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60479
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Distal-less homeobox 3 (DLX3), a member of the Dlx family, plays a crucial role in epithelium, hair, bone, tooth and placental development. DLX3 is expressed during the calcium (Ca2+)-dependent epidermal differentiation process. The calculated molecular weight of DLX3 is 32 kDa. This antibody detects the 32-38 kDa protein in SDS-PAGE. (PMID: 31029881)

实验方案

Product Specific Protocols
WB protocol for DLX3 antibody 13261-3-APDownload protocol
IHC protocol for DLX3 antibody 13261-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,CoIP,IF

J Biol Chem

New insights into the regulation of placental growth factor gene expression by the transcription factors GCM1 and DLX3 in human placenta.

Authors - Yueh-Ho Chiu
View Article
mouseWB,CoIP

Stem Cells

IPO7 promotes odontoblastic differentiation and inhibits osteoblastic differentiation through regulation of RUNX2 expression and translocation

Authors - Yue Zhang
View Article