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验证数据展示

  • IHC staining of mouse testis using 28534-1-AP (same clone as 28534-1-PBS)

    IHC staining of mouse testis using 28534-1-AP (same clone as 28534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKDC antibody included
  • IHC staining of mouse testis using 28534-1-AP (same clone as 28534-1-PBS)

    IHC staining of mouse testis using 28534-1-AP (same clone as 28534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKDC antibody included
  • WB analysis using 28534-1-AP (same clone as 28534-1-PBS)

    WB analysis using 28534-1-AP (same clone as 28534-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28534-1-AP (DNA-PKcs antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 28534-1-AP (same clone as 28534-1-PBS)

    IF Staining of HeLa using 28534-1-AP (same clone as 28534-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28534-1-AP (DNA-PKcs antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

DNA-PKcs Polyclonal antibody, PBS Only

DNA-PKcs Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28534-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DNAPK, DNPK1, PRKDC, DNA-PK catalytic subunit, DNA-dependent protein kinase catalytic subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRKDC Ready-To-Use IHC Kit. PRKDC primary antibody included.

产品信息

28534-1-PBS targets DNA-PKcs in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 DNA-PKcs fusion protein Ag29100 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, DNA-activated, catalytic polypeptide
别名 DNAPK, DNPK1, PRKDC, DNA-PK catalytic subunit, DNA-dependent protein kinase catalytic subunit
计算分子量 469 kDa
观测分子量 350-460 kDa
GenBank蛋白编号NM_006904
基因名称 PRKDC
Gene ID (NCBI) 5591
RRIDAB_2881165
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78527
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.