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DNAI1 Polyclonal antibody

DNAI1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12756-1-AP

Print datasheet

Synonyms

PCD, ICS1, Dynein axonemal intermediate chain 1, CILD1

验证数据展示

  • WB analysis of human brain using 12756-1-AP

    WB analysis of human brain using 12756-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12756-1-AP (DNAI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 12756-1-AP

    WB analysis using 12756-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12756-1-AP (DNAI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 12756-1-AP

    IF Staining of hTERT-RPE1 using 12756-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAI1 antibody (12756-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

经过测试的应用

Positive WB detected inmouse testis tissue, human brain tissue, rat testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12756-1-AP targets DNAI1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen DNAI1 fusion protein Ag3473 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynein, axonemal, intermediate chain 1
Synonyms PCD, ICS1, Dynein axonemal intermediate chain 1, CILD1
Calculated Molecular Weight 699 aa, 79 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC030583
Gene Symbol DNAI1
Gene ID (NCBI) 27019
RRIDAB_10643244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI46
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAI1 antibody 12756-1-APDownload protocol
IF protocol for DNAI1 antibody 12756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Biol

Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.

Authors - Fenglan Dong
View Article
mouseWB,IF

EBioMedicine

CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men

Authors - Hui-Juan Jin
View Article