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验证数据展示

  • WB analysis using 67184-1-Ig (same clone as 67184-1-PBS)

    WB analysis using 67184-1-Ig (same clone as 67184-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67184-1-Ig (same clone as 67184-1-PBS)

    IF Staining of HepG2 using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67184-1-Ig (same clone as 67184-1-PBS)

    WB analysis of HeLa using 67184-1-Ig (same clone as 67184-1-PBS)

    WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

    IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJA1 antibody included
  • IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

    IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJA1 antibody included
  • IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

    IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJA1 antibody included
  • IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

    IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJA1 antibody included
  • IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

    IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJA1 antibody included
  • IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

    IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJA1 antibody included
  • Featured Product
  • KD/KO验证

DNAJA1 Monoclonal antibody, PBS Only

DNAJA1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67184-1-PBS

产品说明书

CloneNo. 1B10A10

宿主/亚型

Mouse / IgM

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HDJ2, hDJ 2, DNAJ2, DjA1, DJ 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy DNAJA1 Ready-To-Use IHC Kit. DNAJA1 primary antibody included.

产品信息

67184-1-PBS targets DNAJA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNAJA1 fusion protein Ag2284 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily A, member 1
别名 HDJ2, hDJ 2, DNAJ2, DjA1, DJ 2
计算分子量 397 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC008182
基因名称 DNAJA1
Gene ID (NCBI) 3301
RRIDAB_2882480
偶联类型 Unconjugated
形式 Liquid
纯化方式Euglobulin precipitation
UNIPROT IDP31689
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.