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  • Featured Product
  • KD/KO Validated

DNAJA1 Monoclonal antibody, PBS Only

DNAJA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10A10

Cat no : 67184-1-PBS

Print datasheet

Synonyms

DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2

验证数据展示

  • WB analysis using 67184-1-Ig (same clone as 67184-1-PBS)

    WB analysis using 67184-1-Ig (same clone as 67184-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67184-1-Ig (same clone as 67184-1-PBS)

    IF Staining of HepG2 using 67184-1-Ig (same clone as 67184-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67184-1-Ig (same clone as 67184-1-PBS)

    WB analysis of HeLa using 67184-1-Ig (same clone as 67184-1-PBS)

    WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy DNAJA1 Ready-To-Use IHC Kit. DNAJA1 primary antibody included.

产品信息

67184-1-PBS targets DNAJA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen DNAJA1 fusion protein Ag2284 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily A, member 1
Synonyms DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2
Calculated Molecular Weight 397 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC008182
Gene Symbol DNAJA1
Gene ID (NCBI) 3301
Conjugate Unconjugated
Form Liquid
Purification MethodEuglobulin precipitation
UNIPROT IDP31689
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.