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  • Featured Product
  • KD/KO Validated

DNAJA1 Monoclonal antibody

DNAJA1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10A10

Cat no : 67184-1-Ig

Print datasheet

Synonyms

DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2

验证数据展示

  • WB analysis using 67184-1-Ig

    WB analysis using 67184-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67184-1-Ig

    IF Staining of HepG2 using 67184-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67184-1-Ig

    WB analysis of HeLa using 67184-1-Ig

    WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy DNAJA1 Ready-To-Use IHC Kit. DNAJA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67184-1-Ig targets DNAJA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen DNAJA1 fusion protein Ag2284 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily A, member 1
Synonyms DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2
Calculated Molecular Weight 397 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC008182
Gene Symbol DNAJA1
Gene ID (NCBI) 3301
RRIDAB_2882480
Conjugate Unconjugated
Form Liquid
Purification MethodEuglobulin precipitation
UNIPROT IDP31689
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

实验方案

Product Specific Protocols
WB protocol for DNAJA1 antibody 67184-1-IgDownload protocol
IF protocol for DNAJA1 antibody 67184-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols