验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Planning an IHC experiment? We recommend our IHCeasy DNAJA1 Ready-To-Use IHC Kit. DNAJA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67184-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67184 targets DNAJA1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | DNAJA1 fusion protein Ag2284 种属同源性预测 |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily A, member 1 |
| 别名 | DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2 |
| 计算分子量 | 397 aa, 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC008182 |
| 基因名称 | DNAJA1 |
| Gene ID (NCBI) | 3301 |
| RRID | AB_2923763 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Euglobulin precipitation |
| UNIPROT ID | P31689 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DNAJA1 antibody CL488-67184 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |