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验证数据展示

  • IF Staining of HepG2 using CL488-67184

    IF Staining of HepG2 using CL488-67184

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DNAJA1 antibody (CL488-67184, Clone: 1B10A10 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL488-67184

    IF Staining of HepG2 using CL488-67184

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DNAJA1 antibody (CL488-67184, Clone: 1B10A10 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DNAJA1 Monoclonal antibody

DNAJA1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67184

Print datasheet

CloneNo. 1B10A10

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy DNAJA1 Ready-To-Use IHC Kit. DNAJA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67184-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67184 targets DNAJA1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen DNAJA1 fusion protein Ag2284 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily A, member 1
Synonyms DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2
Calculated Molecular Weight 397 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC008182
Gene Symbol DNAJA1
Gene ID (NCBI) 3301
RRIDAB_2923763
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodEuglobulin precipitation
UNIPROT IDP31689
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DNAJA1 antibody CL488-67184Download protocol
Standard Protocols
Click here to view our Standard Protocols