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DNAJB12 Polyclonal antibody
DNAJB12 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, A549 cells, human brain tissue, human kidney tissue, human liver tissue, MCF-7 cells, MKN-45 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16780-1-AP targets DNAJB12 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IP, WB, ELISA Application Description |
| Cited Applications | WB, IP, IF, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey |
| Immunogen | DNAJB12 fusion protein Ag10193 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 12 |
| Synonyms | DJ10, DNAJB12 |
| Calculated Molecular Weight | 375 aa, 42 kDa |
| Observed Molecular Weight | 40-42 kDa |
| GenBank Accession Number | BC011812 |
| Gene Symbol | DNAJB12 |
| Gene ID (NCBI) | 54788 |
| RRID | AB_2094404 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NXW2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
| IP protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma. | ||
Nat Commun The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes. | ||
J Cell Biol Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. | ||
PLoS Pathog A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. | ||
J Cell Biol Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication | ||
J Virol The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration. |
