验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, A549 cells, human brain tissue, human kidney tissue, human liver tissue, MCF-7 cells, MKN-45 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16780-1-AP targets DNAJB12 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, monkey |
| 免疫原 | DNAJB12 fusion protein Ag10193 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily B, member 12 |
| 别名 | DJ10 |
| 计算分子量 | 375 aa, 42 kDa |
| 观测分子量 | 40-42 kDa |
| GenBank蛋白编号 | BC011812 |
| 基因名称 | DNAJB12 |
| Gene ID (NCBI) | 54788 |
| RRID | AB_2094404 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NXW2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJB12 (JB12), a ER transmembrane chaperone, specifically interacts with Hsc70 to facilitate ER retention and delivery of membrane proteins for folding or degradation in the ERAD pathway. Hsc70 chaperone activities require the substrate targeting function of DNAJB12. DNAJB12 recruits specific substrates to contact Hsc70 and then stimulates its ATPase activity. (PMID: 34668642)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
| IHC protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
| IP protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma. | ||
Nat Commun The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes. | ||
J Cell Biol Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. | ||
PLoS Pathog A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. | ||
J Cell Biol Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication | ||
J Virol The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration. |
