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DNAJB12 Polyclonal antibody
DNAJB12 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, A549 cells, human brain tissue, human kidney tissue, human liver tissue, MCF-7 cells, MKN-45 cells, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human stomach tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16780-1-AP targets DNAJB12 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IF, IP, ChIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, monkey |
Immunogen | DNAJB12 fusion protein Ag10193 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DnaJ (Hsp40) homolog, subfamily B, member 12 |
Synonyms | DJ10 |
Calculated Molecular Weight | 375 aa, 42 kDa |
Observed Molecular Weight | 40-42 kDa |
GenBank Accession Number | BC011812 |
Gene Symbol | DNAJB12 |
Gene ID (NCBI) | 54788 |
RRID | AB_2094404 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NXW2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
IHC protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
IP protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma. | ||
Nat Commun The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes. | ||
J Cell Biol Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. | ||
PLoS Pathog A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. | ||
J Cell Biol Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication | ||
J Virol The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration. |