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  • Featured Product
  • KD/KO Validated

Biotin-conjugated DNAJB12 Polyclonal antibody

DNAJB12 Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-16780

Print datasheet

Synonyms

DJ10, DKFZp586B2023, DNAJB12

验证数据展示

  • IHC staining of human colon cancer using Biotin-16780

    IHC staining of human colon cancer using Biotin-16780

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16780 (DNAJB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using Biotin-16780

    IHC staining of human colon cancer using Biotin-16780

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16780 (DNAJB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-16780 targets DNAJB12 in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC Application Description
Tested Reactivity human, mouse, rat
Immunogen DNAJB12 fusion protein Ag10193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 12
Synonyms DJ10, DKFZp586B2023, DNAJB12
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC011812
Gene Symbol DNAJB12
Gene ID (NCBI) 54788
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXW2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Biotin DNAJB12 antibody Biotin-16780Download protocol
Standard Protocols
Click here to view our Standard Protocols