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验证数据展示

  • IHC staining of human colon cancer using Biotin-16780

    IHC staining of human colon cancer using Biotin-16780

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16780 (DNAJB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using Biotin-16780

    IHC staining of human colon cancer using Biotin-16780

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16780 (DNAJB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

Biotin-conjugated DNAJB12 Polyclonal antibody

DNAJB12 Polyclonal Antibody for IHC
Cat No. Biotin-16780

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC

别名

DJ10, DKFZp586B2023, DNAJB12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-16780 targets DNAJB12 in IHC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC Application Description
经测试反应性 human, mouse, rat
免疫原 DNAJB12 fusion protein Ag10193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 12
别名 DJ10, DKFZp586B2023, DNAJB12
计算分子量 375 aa, 42 kDa
观测分子量 40-42 kDa
GenBank蛋白编号BC011812
基因名称 DNAJB12
Gene ID (NCBI) 54788
RRIDAB_2934329
偶联类型 Biotin
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXW2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Biotin DNAJB12 antibody Biotin-16780Download protocol
Standard Protocols
Click here to view our Standard Protocols