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DNAJB3 Polyclonal antibody

DNAJB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17177-1-AP

Print datasheet

Synonyms

DNAJB3, HCG3



经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human kidney tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17177-1-AP targets DNAJB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DNAJB3 fusion protein Ag10878 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 3
Synonyms DNAJB3, HCG3
Calculated Molecular Weight 145 aa, 17 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC024013
Gene Symbol DNAJB3
Gene ID (NCBI) 414061
RRIDAB_2094420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWF6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJB3 antibody 17177-1-APDownload protocol
IHC protocol for DNAJB3 antibody 17177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

PMID: 26400768

Authors - Mohamed Abu-Farha
mouseWB

Sci Rep

Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone.

Authors - Abdoulaye Diane
  • KD Validated
humanWB,IHC

PLoS One

DNAJB3/HSP-40 Cochaperone Is Downregulated in Obese Humans and Is Restored by Physical Exercise.

Authors - Abubaker Jehad J
mouseWB

Int J Mol Sci

Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice.

Authors - Ryo Mitsugi
humanWB,IHC

Lipids Health Dis

Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue.

Authors - Ali Tiss
mouseWB

Sci Rep

DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation.

Authors - Abdelilah Arredouani
  • KD Validated