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DNAJB3 Polyclonal antibody
DNAJB3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IHC detected in | mouse testis tissue, human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 3 publications below |
WB | See 8 publications below |
IHC | See 2 publications below |
产品信息
17177-1-AP targets DNAJB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | DNAJB3 fusion protein Ag10878 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DnaJ (Hsp40) homolog, subfamily B, member 3 |
Synonyms | DNAJB3, HCG3 |
Calculated Molecular Weight | 145 aa, 17 kDa |
Observed Molecular Weight | 28-30 kDa |
GenBank Accession Number | BC024013 |
Gene Symbol | DNAJB3 |
Gene ID (NCBI) | 414061 |
RRID | AB_2094420 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8WWF6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for DNAJB3 antibody 17177-1-AP | Download protocol |
IHC protocol for DNAJB3 antibody 17177-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Rep Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone.
| ||
PLoS One DNAJB3/HSP-40 Cochaperone Is Downregulated in Obese Humans and Is Restored by Physical Exercise. | ||
Int J Mol Sci Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice. | ||
Lipids Health Dis Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue. | ||
Sci Rep DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation.
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