DNAJC2/MPP11 Polyclonal antibody

DNAJC2/MPP11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11971-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

DNAJC2, DnaJ homolog subfamily C member 2, N-terminally processed, ZRF1, MPP11, MPHOSPH11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCaco-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, mouse liver tissue, PC-3 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DNAJC2/ZRF1 Ready-To-Use IHC Kit. DNAJC2/ZRF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11971-1-AP targets DNAJC2/MPP11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 DNAJC2/MPP11 fusion protein Ag2594 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 2
别名 DNAJC2, DnaJ homolog subfamily C member 2, N-terminally processed, ZRF1, MPP11, MPHOSPH11
计算分子量 72 kDa
观测分子量 66-75 kDa
GenBank蛋白编号BC056682
基因名称 DNAJC2
Gene ID (NCBI) 27000
RRIDAB_10695893
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99543
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
IHC protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
IF protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
IP protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins.

Authors - Guiyou Tian
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem
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