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DNAJC2/MPP11 Polyclonal antibody

DNAJC2/MPP11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11971-1-AP

Print datasheet

Synonyms

DNAJC2, DnaJ homolog subfamily C member 2, N-terminally processed, ZRF1, MPP11, MPHOSPH11

验证数据展示

  • IF Staining of HepG2 using 11971-1-AP

    IF Staining of HepG2 using 11971-1-AP

    Immunofluorescent analysis of HepG2 cells, using DNAJC2 antibody 11971-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse liver using 11971-1-AP

    IP experiment of mouse liver using 11971-1-AP

    IP result of anti-DNAJC2/MPP11 (IP:11971-1-AP, 4ug; Detection:11971-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

  • IHC staining of human liver cancer using 11971-1-AP

    IHC staining of human liver cancer using 11971-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11971-1-AP (DNAJC2/MPP11 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DNAJC2/ZRF1 antibody included
  • IHC staining of human liver cancer using 11971-1-AP

    IHC staining of human liver cancer using 11971-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11971-1-AP (DNAJC2/MPP11 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DNAJC2/ZRF1 antibody included
  • WB analysis using 11971-1-AP

    WB analysis using 11971-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCaco-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, mouse liver tissue, PC-3 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DNAJC2/ZRF1 Ready-To-Use IHC Kit. DNAJC2/ZRF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11971-1-AP targets DNAJC2/MPP11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DNAJC2/MPP11 fusion protein Ag2594 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C, member 2
Synonyms DNAJC2, DnaJ homolog subfamily C member 2, N-terminally processed, ZRF1, MPP11, MPHOSPH11
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 66-75 kDa
GenBank Accession NumberBC056682
Gene Symbol DNAJC2
Gene ID (NCBI) 27000
RRIDAB_10695893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
IHC protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
IF protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
IP protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins.

Authors - Guiyou Tian
View Article
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem
View Article