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DNAJC21 Monoclonal antibody, PBS Only

DNAJC21 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8A9

Cat no : 68343-1-PBS

Print datasheet

Synonyms

DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3

验证数据展示

  • WB analysis using 68343-1-Ig (same clone as 68343-1-PBS)

    WB analysis using 68343-1-Ig (same clone as 68343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68343-1-Ig (DNAJC21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68343-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68343-1-Ig (same clone as 68343-1-PBS)

    IF Staining of U2OS using 68343-1-Ig (same clone as 68343-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC21 antibody (68343-1-Ig, Clone: 2F8A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68343-1-PBS in a different storage buffer formulation.

产品信息

68343-1-PBS targets DNAJC21 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen DNAJC21 fusion protein Ag20003 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily C, member 21
Synonyms DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3
Calculated Molecular Weight 531 aa, 58 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC107577
Gene Symbol DNAJC21
Gene ID (NCBI) 134218
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5F1R6
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)