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验证数据展示

  • WB analysis using 68343-1-Ig

    WB analysis using 68343-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68343-1-Ig (DNAJC21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of U2OS using 68343-1-Ig

    IF Staining of U2OS using 68343-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC21 antibody (68343-1-Ig, Clone: 2F8A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DNAJC21 Monoclonal antibody

DNAJC21 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68343-1-Ig

产品说明书

CloneNo. 2F8A9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68343-1-Ig targets DNAJC21 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 DNAJC21 fusion protein Ag20003 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 21
别名 DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3
计算分子量 531 aa, 58 kDa
观测分子量 70 kDa
GenBank蛋白编号BC107577
基因名称 DNAJC21
Gene ID (NCBI) 134218
RRIDAB_3085067
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ5F1R6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)

实验方案

Product Specific Protocols
WB protocol for DNAJC21 antibody 68343-1-IgDownload protocol
IF protocol for DNAJC21 antibody 68343-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols