验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
For other applications, we recommend the unconjugated version of this antibody, 68343-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68343 targets DNAJC21 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | DNAJC21 fusion protein Ag20003 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | DnaJ (Hsp40) homolog, subfamily C, member 21 |
| Synonyms | DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3 |
| Calculated Molecular Weight | 531 aa, 58 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC107577 |
| Gene Symbol | DNAJC21 |
| Gene ID (NCBI) | 134218 |
| RRID | AB_3672996 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q5F1R6 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DNAJC21 antibody CL488-68343 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |