DNASE1L3 Monoclonal antibody
DNASE1L3 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A4D4
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, U2OS cells, Daudi cells, A431 cells, LnCaP cells, Jurkat cells, K-562 cells, HepG2 cells, Raji cells |
| Positive IHC detected in | mouse liver tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67041-1-Ig targets DNASE1L3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Immunogen | DNASE1L3 fusion protein Ag28187 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | deoxyribonuclease I-like 3 |
| Synonyms | Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD |
| Calculated Molecular Weight | 305 aa, 36 kDa |
| Observed Molecular Weight | 33-38 kDa |
| GenBank Accession Number | BC015831 |
| Gene Symbol | DNASE1L3 |
| Gene ID (NCBI) | 1776 |
| RRID | AB_2882355 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13609 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+- dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences (PMID:157967140). It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3′-OH/5′-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin (PMID:9665719). In addition, it translocates from the endoplasmic reticulum to the nucleus during apoptosis (PMID: 23229555). DNASE1L3 can be located either in the cytoplasm or in the nucleus.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNASE1L3 antibody 67041-1-Ig | Download protocol |
| IHC protocol for DNASE1L3 antibody 67041-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Deoxyribonuclease 1-like 3 may be a potential prognostic biomarker associated with immune infiltration in colon cancer. |
