• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HepG2 using CL594-67041

    FC experiment of HepG2 using CL594-67041

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DNASE1L3 (CL594-67041, Clone:1A4D4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL594-67041

    IF Staining of HepG2 using CL594-67041

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 DNASE1L3 antibody (CL594-67041, Clone: 1A4D4 ) at dilution of 1:200, CL488-Phalloidin (green).

CoraLite®594-conjugated DNASE1L3 Monoclonal antibody

DNASE1L3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67041

产品说明书

CloneNo. 1A4D4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

DNase gamma, DNAS1L3, DHP2, Deoxyribonuclease I-like 3, Deoxyribonuclease gamma

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67041-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67041 targets DNASE1L3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 DNASE1L3 fusion protein Ag28187 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 deoxyribonuclease I-like 3
别名 DNase gamma, DNAS1L3, DHP2, Deoxyribonuclease I-like 3, Deoxyribonuclease gamma
计算分子量 305 aa, 36 kDa
观测分子量 33-38 kDa
GenBank蛋白编号BC015831
基因名称 DNASE1L3
Gene ID (NCBI) 1776
RRIDAB_2920078
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13609
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+- dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences [PMID:15796714]. It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3′-OH/5′-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin [PMID:9665719]. In addition, it has been suggested to be involved in apoptotic DNA fragmentation of thymocytes' neuronal PC12 cells, C2C12 myoblasts and of cell lines expressing the recombinant nuclease [PMID: 7957253, 9307016]

实验方案

Product Specific Protocols
IF protocol for CL594 DNASE1L3 antibody CL594-67041Download protocol
FC protocol for CL594 DNASE1L3 antibody CL594-67041Download protocol
Standard Protocols
Click here to view our Standard Protocols