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DRP1 (C-terminal) Polyclonal antibody
DRP1 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
240
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, HeLa cells, A549 cells, rat brain tissue, DU 145 cells, HCT 116 cells, HepG2 cells, NIH/3T3 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12957-1-AP targets DRP1 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey, chicken, zebrafish, bovine, hamster, duck |
Immunogen | DRP1 (C-terminal) fusion protein Ag3644 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | dynamin 1-like |
Synonyms | DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L |
Calculated Molecular Weight | 736 aa, 82 kDa |
Observed Molecular Weight | 75-80 kDa |
GenBank Accession Number | BC024590 |
Gene Symbol | DRP1 |
Gene ID (NCBI) | 10059 |
RRID | AB_2093525 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00429 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
IHC protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
IF protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
IP protocol for DRP1 (C-terminal) antibody 12957-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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