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验证数据展示

  • FC experiment of HeLa using CL647-12957

    FC experiment of HeLa using CL647-12957

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human DRP1 (C-terminal) (CL647-12957) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL647-12957

    IF Staining of HeLa using CL647-12957

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 DRP1 (C-terminal) antibody (CL647-12957) at dilution of 1:200.

  • IF Staining of HeLa using CL647-12957

    IF Staining of HeLa using CL647-12957

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 DRP1 (C-terminal) antibody (CL647-12957) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated DRP1 (C-terminal) Polyclonal antibody

DRP1 (C-terminal) Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-12957

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647

Size: 
100 μL

¥3200/ 100 μL

浓度: 1000 ug/ml

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12957-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-12957 targets DRP1 (C-terminal) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen DRP1 (C-terminal) fusion protein Ag3644 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynamin 1-like
Synonyms DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L
Calculated Molecular Weight 736 aa, 82 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC024590
Gene Symbol DRP1
Gene ID (NCBI) 10059
RRIDAB_2934878
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00429
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 DRP1 (C-terminal) antibody CL647-12957Download protocol
FC protocol for CL Plus 647 DRP1 (C-terminal) antibody CL647-12957Download protocol
Standard Protocols
Click here to view our Standard Protocols