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CoraLite® Plus 647-conjugated DRP1 (C-terminal) Polyclonal antibody
DRP1 (C-terminal) Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 12957-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-12957 targets DRP1 (C-terminal) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | DRP1 (C-terminal) fusion protein Ag3644 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | dynamin 1-like |
Synonyms | DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L |
Calculated Molecular Weight | 736 aa, 82 kDa |
Observed Molecular Weight | 75-80 kDa |
GenBank Accession Number | BC024590 |
Gene Symbol | DRP1 |
Gene ID (NCBI) | 10059 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00429 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 DRP1 (C-terminal) antibody CL647-12957 | Download protocol |
FC protocol for CL Plus 647 DRP1 (C-terminal) antibody CL647-12957 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |