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验证数据展示

  • FC experiment of HeLa using CL647-12957

    FC experiment of HeLa using CL647-12957

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human DRP1 (C-terminal) (CL647-12957) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL647-12957

    IF Staining of HeLa using CL647-12957

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 DRP1 (C-terminal) antibody (CL647-12957) at dilution of 1:200.

  • IF Staining of HeLa using CL647-12957

    IF Staining of HeLa using CL647-12957

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 DRP1 (C-terminal) antibody (CL647-12957) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated DRP1 (C-terminal) Polyclonal antibody

DRP1 (C-terminal) Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-12957

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12957-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-12957 targets DRP1 (C-terminal) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 DRP1 (C-terminal) fusion protein Ag3644 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynamin 1-like
别名 DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L
计算分子量 736 aa, 82 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC024590
基因名称 DRP1
Gene ID (NCBI) 10059
RRIDAB_2934878
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00429
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 DRP1 (C-terminal) antibody CL647-12957Download protocol
FC protocol for CL Plus 647 DRP1 (C-terminal) antibody CL647-12957Download protocol
Standard Protocols
Click here to view our Standard Protocols