DOCK180 Polyclonal antibody
DOCK180 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, LNCaP cells, MCF-7 cells, A431 cells, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
产品信息
The immunogen of 23421-1-AP is DOCK180 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse, zebrafish |
Immunogen | DOCK180 fusion protein Ag18069 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | dedicator of cytokinesis 1 |
Synonyms | ced5, dedicator of cytokinesis 1, DOCK1, DOCK180 |
Calculated Molecular Weight | 1865 aa, 215 kDa |
Observed Molecular Weight | 215 kDa |
GenBank Accession Number | BC146857 |
Gene Symbol | DOCK1 |
Gene ID (NCBI) | 1793 |
RRID | AB_2879276 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14185 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for DOCK180 antibody 23421-1-AP | Download protocol |
IHC protocol for DOCK180 antibody 23421-1-AP | Download protocol |
IF protocol for DOCK180 antibody 23421-1-AP | Download protocol |
IP protocol for DOCK180 antibody 23421-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Diabetes Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing. | ||
Cell Death Dis Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways. | ||
bioRxiv Dock1 acts cell-autonomously in Schwann cells to regulate the development, maintenance, and repair of peripheral myelin | ||
Biomaterials Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs | ||
Exp Cell Res Acetylation of ELMO1 correlates with Rac1 activity and colorectal cancer progress |