DOCK180 Polyclonal antibody

DOCK180 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23421-1-AP

Print datasheet

Synonyms

ced5, dedicator of cytokinesis 1, DOCK1, DOCK180



经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, MCF-7 cells, A431 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

The immunogen of 23421-1-AP is DOCK180 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish
Immunogen DOCK180 fusion protein Ag18069 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dedicator of cytokinesis 1
Synonyms ced5, dedicator of cytokinesis 1, DOCK1, DOCK180
Calculated Molecular Weight 1865 aa, 215 kDa
Observed Molecular Weight 215 kDa
GenBank Accession NumberBC146857
Gene Symbol DOCK1
Gene ID (NCBI) 1793
RRIDAB_2879276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14185
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DOCK180 antibody 23421-1-APDownload protocol
IHC protocol for DOCK180 antibody 23421-1-APDownload protocol
IF protocol for DOCK180 antibody 23421-1-APDownload protocol
IP protocol for DOCK180 antibody 23421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Diabetes

Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing.

Authors - Hua Qu
mouseWB

Cell Death Dis

Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways.

Authors - Xujuan Sun
zebrafishWB

bioRxiv

Dock1 acts cell-autonomously in Schwann cells to regulate the development, maintenance, and repair of peripheral myelin

Authors - Ryan A Doan
humanWB

Biomaterials

Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs

Authors - Anna L Kersey
humanWB

Exp Cell Res

Acetylation of ELMO1 correlates with Rac1 activity and colorectal cancer progress

Authors - Chuangkun Li