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验证数据展示

  • WB analysis using 68151-1-Ig

    WB analysis using 68151-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 68151-1-Ig

    WB analysis using 68151-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of LNCaP using 68151-1-Ig

    IF Staining of LNCaP using 68151-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using DOHH antibody (68151-1-Ig, Clone: 1H5H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

DOHH Monoclonal antibody

DOHH Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68151-1-Ig

产品说明书

CloneNo. 1H5H7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29, 1H5H7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IF/ICC detected inLNCaP cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68151-1-Ig targets DOHH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DOHH fusion protein Ag13041 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deoxyhypusine hydroxylase/monooxygenase
别名 HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29, 1H5H7
计算分子量 33 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC002817
基因名称 DOHH
Gene ID (NCBI) 83475
RRIDAB_2935259
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BU89
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).

实验方案

Product Specific Protocols
WB protocol for DOHH antibody 68151-1-IgDownload protocol
IF protocol for DOHH antibody 68151-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols