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验证数据展示

  • WB analysis using 68151-1-Ig

    WB analysis using 68151-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 68151-1-Ig

    WB analysis using 68151-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of LNCaP using 68151-1-Ig

    IF Staining of LNCaP using 68151-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using DOHH antibody (68151-1-Ig, Clone: 1H5H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

DOHH Monoclonal antibody

DOHH Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68151-1-Ig

Print datasheet

CloneNo. 1H5H7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29, 1H5H7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IF/ICC detected inLNCaP cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68151-1-Ig targets DOHH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DOHH fusion protein Ag13041 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name deoxyhypusine hydroxylase/monooxygenase
Synonyms HLRC1, HEAT-like repeat-containing protein 1, hDOHH, EC:1.14.99.29, 1H5H7
Calculated Molecular Weight 33 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC002817
Gene Symbol DOHH
Gene ID (NCBI) 83475
RRIDAB_2935259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BU89
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).

实验方案

Product Specific Protocols
WB protocol for DOHH antibody 68151-1-IgDownload protocol
IF protocol for DOHH antibody 68151-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols