• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of LNCaP using CL488-68151

    IF Staining of LNCaP using CL488-68151

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using CoraLite® Plus 488 DOHH antibody (CL488-68151, Clone: 1H5H7 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated DOHH Monoclonal antibody

DOHH Monoclonal Antibody for IF/ICC
Cat No. CL488-68151

产品说明书

CloneNo. 1H5H7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inLNCaP cells
For other applications, we recommend the unconjugated version of this antibody, 68151-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68151 targets DOHH in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 DOHH fusion protein Ag13041 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deoxyhypusine hydroxylase/monooxygenase
别名
计算分子量 33 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC002817
基因名称 DOHH
Gene ID (NCBI) 83475
RRIDAB_3672975
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BU89
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DOHH antibody CL488-68151Download protocol
Standard Protocols
Click here to view our Standard Protocols