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DPEP1 Monoclonal antibody

DPEP1 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig, rabbit

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B4F4

Cat No : 68081-1-Ig

Print datasheet

Synonyms

Dehydropeptidase I, Dipeptidase 1, dipeptidase 1 (renal), DPEP1, hRDP, MBD1, MDP, Microsomal dipeptidase, RDP, Renal dipeptidase

验证数据展示

  • WB analysis of human testis using 68081-1-Ig

    WB analysis of human testis using 68081-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 68081-1-Ig

    IHC staining of human kidney using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 68081-1-Ig

    WB analysis of HepG2 using 68081-1-Ig

    WB result of DPEP1 antibody (68081-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPEP1 transfected HepG2 cells.

  • IF Staining of mouse kidney using 68081-1-Ig

    IF Staining of mouse kidney using 68081-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DPEP1 antibody (68081-1-Ig, Clone: 2B4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman testis tissue, rabbit kidney tissue, pig pancreas tissue, rat pancreas tissue, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68081-1-Ig targets DPEP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat, Pig, rabbit
Immunogen DPEP1 fusion protein Ag14591 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name dipeptidase 1 (renal)
Synonyms Dehydropeptidase I, Dipeptidase 1, dipeptidase 1 (renal), DPEP1, hRDP, MBD1, MDP, Microsomal dipeptidase, RDP, Renal dipeptidase
Calculated Molecular Weight 411 aa, 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC017023
Gene Symbol DPEP1
Gene ID (NCBI) 1800
RRIDAB_2918818
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DPEP1 antibody 68081-1-IgDownload protocol
IHC protocol for DPEP1 antibody 68081-1-IgDownload protocol
IF protocol for DPEP1 antibody 68081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols