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验证数据展示

  • WB analysis using 26219-1-AP

    WB analysis using 26219-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26219-1-AP (DRC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 26219-1-AP

    IF Staining of hTERT-RPE1 using 26219-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DRC1 antibody (26219-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

DRC1 Polyclonal antibody

DRC1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26219-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

C2orf39, Dynein regulatory complex protein 1, Coiled-coil domain-containing protein 164, CCDC164

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26219-1-AP targets DRC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DRC1 fusion protein Ag24113 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 2 open reading frame 39
别名 C2orf39, Dynein regulatory complex protein 1, Coiled-coil domain-containing protein 164, CCDC164
观测分子量 87 kDa
GenBank蛋白编号BC009488
基因名称 C2orf39/DRC1
Gene ID (NCBI) 92749
RRIDAB_3085848
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MC2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DRC1, also known as C2orf39, CCDC164, has 3 coiled-coil motifs in its N-terminal half and an additional coiled-coil motif near its C terminus. DRC1 is a subunit of the nexin-dynein regulatory complex, an axonemal structure critical for the regulation of ciliary dynein motors. DRC1 is predominantly expressed in the lung, brain, and prostate (PMID: 23354437). DRC1 deficiency disrupts the assembly of the nexin-dynein regulatory complex, causing ciliary dyskinesia(PMID: 23354437, 34815526).

实验方案

Product Specific Protocols
WB protocol for DRC1 antibody 26219-1-APDownload protocol
IF protocol for DRC1 antibody 26219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols