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验证数据展示

  • IF Staining of U2OS using CL488-67164

    IF Staining of U2OS using CL488-67164

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 DRG2 antibody (CL488-67164, Clone: 3A3E1 ) at dilution of 1:200.

  • FC experiment of U2OS using CL488-67164

    FC experiment of U2OS using CL488-67164

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DRG2 Monoclonal antibody (CL488-67164, Clone:3A3E1)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated DRG2 Monoclonal antibody

DRG2 Monoclonal Antibody for IF/ICC
Cat No. CL488-67164

产品说明书

CloneNo. 3A3E1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

IF/ICC

别名

TRAFAC GTPase DRG2, EC:3.6.5.-, DRG-2, DRG 2, Developmentally-regulated GTP-binding protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 67164-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67164 targets DRG2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig
免疫原 DRG2 fusion protein Ag6878 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 developmentally regulated GTP binding protein 2
别名 TRAFAC GTPase DRG2, EC:3.6.5.-, DRG-2, DRG 2, Developmentally-regulated GTP-binding protein 2
计算分子量 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC000493
基因名称 DRG2
Gene ID (NCBI) 1819
RRIDAB_3672938
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55039
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DRG2 antibody CL488-67164Download protocol
FC protocol for CL Plus 488 DRG2 antibody CL488-67164Download protocol
Standard Protocols
Click here to view our Standard Protocols