DSG1 Recombinant antibody
DSG1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
241093G11
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, rat skin tissue |
| Positive IHC detected in | mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DSG1 Ready-To-Use IHC Kit. DSG1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 84053-6-RR is DSG1 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | human, rat |
| Immunogen | DSG1 fusion protein Ag20184 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | desmoglein 1 |
| Synonyms | Desmosomal glycoprotein 1, Desmoglein-1, desmoglein 1, CDHF4, Cadherin family member 4 |
| Calculated Molecular Weight | 1049 aa, 114 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | BC153001 |
| Gene Symbol | DSG1 |
| Gene ID (NCBI) | 1828 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q02413 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and is crucial in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder to striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DSG1 antibody 84053-6-RR | Download protocol |
| IHC protocol for DSG1 antibody 84053-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |