验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293 cells, A431 cells, A549 cells, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21880-1-AP targets DSG2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DSG2 fusion protein Ag16393 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | desmoglein 2 |
| 别名 | Desmoglein-2, Desmoglein 2, CDHF5, Cadherin family member 5, ARVD10 |
| 计算分子量 | 1118 aa, 122 kDa |
| 观测分子量 | 145-150 kDa |
| GenBank蛋白编号 | BC099655 |
| 基因名称 | DSG2 |
| Gene ID (NCBI) | 1829 |
| RRID | AB_10836933 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14126 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DSG2 antibody 21880-1-AP | Download protocol |
| IHC protocol for DSG2 antibody 21880-1-AP | Download protocol |
| IF protocol for DSG2 antibody 21880-1-AP | Download protocol |
| IP protocol for DSG2 antibody 21880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep O-glycan initiation directs distinct biological pathways and controls epithelial differentiation. | ||
Virol Sin Desmoglein 2 (DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia.
| ||
Helicobacter Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact. | ||
Mol Biol Cell Plakophilin 3 and Par3 Facilitate Desmosomes' association with the Apical Junctional Complex. | ||
Food Chem Toxicol Sodium fluoride and sulfur dioxide affected male reproduction by disturbing blood-testis barrier in mice. | ||
Microscopy (Oxf) Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes. |
