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验证数据展示

  • IF Staining of A431 using CL488-21880

    IF Staining of A431 using CL488-21880

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 DSG2 antibody (CL488-21880) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated DSG2 Polyclonal antibody

DSG2 Polyclonal Antibody for IF/ICC
Cat No. CL488-21880

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Desmoglein-2, Desmoglein 2, CDHF5, Cadherin family member 5, ARVD10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy DSG2 Ready-To-Use IHC Kit. DSG2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21880-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21880 targets DSG2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 DSG2 fusion protein Ag16393 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 desmoglein 2
别名 Desmoglein-2, Desmoglein 2, CDHF5, Cadherin family member 5, ARVD10
计算分子量 1118 aa, 122 kDa
观测分子量 145-150 kDa
GenBank蛋白编号BC099655
基因名称 DSG2
Gene ID (NCBI) 1829
RRIDAB_3084058
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14126
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DSG2 antibody CL488-21880Download protocol
Standard Protocols
Click here to view our Standard Protocols