• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68515-1-Ig

    WB analysis using 68515-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68515-1-Ig (DSG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

DSG2 Monoclonal antibody

DSG2 Monoclonal Antibody for WB, ELISA
Cat No. 68515-1-Ig

产品说明书

CloneNo. 1B8C3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, K-562 cells, A549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DSG2 Ready-To-Use IHC Kit. DSG2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68515-1-Ig targets DSG2 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 DSG2 fusion protein Ag20633 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 desmoglein 2
别名 ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC
计算分子量 1118 aa, 122 kDa
观测分子量145-150 kDa
GenBank蛋白编号BC099655
基因名称 DSG2
Gene ID (NCBI) 1829
RRIDAB_3085224
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14126
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).

实验方案

Product Specific Protocols
WB protocol for DSG2 antibody 68515-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols