验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells, A431 cells, MCF-7 cells, HEK-293 cells |
Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68364-1-Ig targets DSP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human, mouse |
免疫原 | DSP fusion protein Ag30499 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | desmoplakin |
别名 | desmoplakin, DP, DPI, DPII, DSP |
计算分子量 | 2871 aa, 332 kDa |
观测分子量 | 240-330 kDa |
GenBank蛋白编号 | BC140802 |
基因名称 | DSP |
Gene ID (NCBI) | 1832 |
RRID | AB_3085085 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P15924 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)
实验方案
Product Specific Protocols | |
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WB protocol for DSP antibody 68364-1-Ig | Download protocol |
IHC protocol for DSP antibody 68364-1-Ig | Download protocol |
IF protocol for DSP antibody 68364-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |