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验证数据展示

  • WB analysis using 68364-1-Ig

    WB analysis using 68364-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68364-1-Ig (DSP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 68364-1-Ig

    IHC staining of mouse heart using 68364-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 68364-1-Ig

    IF Staining of A431 using 68364-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSP antibody (68364-1-Ig, Clone: 1B10D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DSP Monoclonal antibody

DSP Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68364-1-Ig

Print datasheet

CloneNo. 1B10D4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

desmoplakin, DP, DPI, DPII, DSP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, A431 cells, MCF-7 cells, HEK-293 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68364-1-Ig targets DSP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen DSP fusion protein Ag30499 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name desmoplakin
Synonyms desmoplakin, DP, DPI, DPII, DSP
Calculated Molecular Weight 2871 aa, 332 kDa
Observed Molecular Weight 240-330 kDa
GenBank Accession NumberBC140802
Gene Symbol DSP
Gene ID (NCBI) 1832
RRIDAB_3085085
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15924
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

实验方案

Product Specific Protocols
WB protocol for DSP antibody 68364-1-IgDownload protocol
IHC protocol for DSP antibody 68364-1-IgDownload protocol
IF protocol for DSP antibody 68364-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols