• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DSP Monoclonal antibody, PBS Only

DSP Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1B10D4

Cat No : 68364-1-PBS

Print datasheet

Synonyms

desmoplakin, DP, DPI, DPII, DSP

验证数据展示

  • WB analysis using 68364-1-Ig (same clone as 68364-1-PBS)

    WB analysis using 68364-1-Ig (same clone as 68364-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68364-1-Ig (DSP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

    IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68364-1-Ig (same clone as 68364-1-PBS)

    IF Staining of A431 using 68364-1-Ig (same clone as 68364-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSP antibody (68364-1-Ig, Clone: 1B10D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

产品信息

68364-1-PBS targets DSP in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse
Immunogen DSP fusion protein Ag30499 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name desmoplakin
Synonyms desmoplakin, DP, DPI, DPII, DSP
Calculated Molecular Weight 2871 aa, 332 kDa
Observed Molecular Weight 240-330 kDa
GenBank Accession NumberBC140802
Gene Symbol DSP
Gene ID (NCBI) 1832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15924
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)